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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant purpose in marketing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two supplemental splice variants were described but in-depth nucleotide sequence data isn't yet available.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded from the antibody.
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This guidebook outlines further more attainable optimization of mobile and immunoassay parameters to make sure the very best results are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no website longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is particular.
Goal Information Cyclin E belongs for the hugely conserved cyclin household, whose customers are characterised by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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